CD1a (Pan T Cell Marker)

CD3 is the pan–T‑cell marker; it forms part of the T‑cell receptor complex and is expressed on all mature T lymphocytes. It’s routinely used in immunophenotyping and flow cytometry to identify and quantify T cells and evaluate T‑cell disorders. CD2 and CD5 are additional T‑lineage markers but are less specific than CD3.

CD1a is expressed primarily on Langerhans cells in the epidermis and on certain dendritic cells (especially myeloid and thymic dendritic cells). It’s also present on cortical thymocytes and on some monocyte-derived dendritic cell populations. CD1a is not typically found on B cells or mature peripheral blood T cells. It presents lipid antigens to T cells.

Yes. CD3 is a core component of the T‑cell receptor complex and is widely used as a pan‑T‑cell marker because it is expressed on essentially all mature T lymphocytes. It identifies T cells in flow cytometry and immunohistochemistry. Exceptions: immature thymocytes may show cytoplasmic but not surface CD3, and some rare leukemias or aberrant T‑cell populations can lack CD3.

CD3 is the pan‑T cell marker. It is expressed on all mature T lymphocytes as part of the T‑cell receptor complex and is routinely used to identify and count total T cells. CD3 staining distinguishes T cells from B cells and other leukocytes; CD4 and CD8 identify T‑cell subsets, not the entire T‑cell population.

T cells are defined by CD3, a pan–T‑cell marker. Subsets include CD4+ helper T cells and CD8+ cytotoxic T cells. Other common T‑cell markers are CD2, CD5, CD7 and costimulatory CD28. Regulatory T cells express CD25 and FOXP3 (intracellular). Memory/naïve markers include CD45RO and CD45RA; activation markers include CD69 and CD25.

CD3 is a protein complex on T lymphocytes that associates with the T‑cell receptor and transmits activation signals, used to identify and quantify T cells. CD19 is a B‑cell surface protein expressed through most stages of B‑cell development, used to detect and monitor B‑cell populations and guide therapies targeting B cells. Both markers are routinely measured by flow cytometry.